Figure 3

Quantification of neutral lipids and phospholipids after PCSK9 silencing. (A,B) Cholesterol, triacyl glycerol (TAG), fatty acid (FA) diacyl glycerol (DAG), phosphatidylcholine (PC), -serine (PS), -inositol (PI) and -ethanolamine (PE) were measured in (A) HepG2 and (B) Huh7 after transfection with PCSK9 siRNA or the control for 144 h. Unpaired t-test where each siRNA PCSK9 group was compared to the CTR group alone, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 (n = 3, with 3 technical replicates within each biological replicate). (C) Cells were transfected with siRNA (si1 and si2) for 72 h then fed with [1-¹⁴C] acetate. Lipid extraction was performed 1, 2 and 4 h after [1-¹⁴C] acetate feeding and radiolabeled PC, PE, PS and PI amounts were measured at each time point. Average values were obtained by combining values from both PCSK9 siRNAs (n = 1, with 3 technical replicates within each condition).