FIGURE

Figure 2

ID

ZDB-FIG-230110-28

Publication

Alannan et al., 2022 - Targeting PCSK9 in Liver Cancer Cells Triggers Metabolic Exhaustion and Cell Death by Ferroptosis

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Figure 2

Effect of PCSK9 silencing by siRNA on liver cancer cell proliferation. (A) Validation of the depletion of PCSK9 by siRNA silencing. Cell extracts were prepared 24, 48 and 72 h after transfection with a control siRNA (siCTR) or targeting PCSK9 (si1 and si2) of Huh6, Huh7 or HepG2 cells. A total of 40 �g of cell proteins were loaded per lane on an SDS-PAGE. After electrophoresis and transfer, the membrane was analyzed by Western blot using anti-PCSK9 and GAPDH antibodies for comparison of loading. The two bands observed for PCSK9 correspond to the pro-PCSK9 (74 kDa) and cleaved PCSK9 (60 kDa). (B) Evaluation of liver cancer cell proliferation by MTS after transfection with si1 and si2 in comparison with siCTR. Two-way ANOVA test, *** p < 0.001; **** p < 0.0001 (n = 3, with 3 technical replicates within each biological replicate).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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