The antipsychotic drug Risperidone ameliorates the social behavior impairment of setd5 adult mutant zebrafish. (a) Set-up of zebrafish adult fish during the two rounds of social preference test on the fish (white) included in the central arena. Zone 1 and zone 2, highlighted in red, indicates the areas of the arena near to the first stimulus (purple) and social novelty stimulus (blue) added at round 2. (b,c) Time spent by setd5+/+ and setd5+/− adult fish in zone 1 and zone 2, in both rounds of social preference test, for untreated (b) and Risperidone-treated fishes (c). (d) Set-up of the social behavior test, evidencing the distance between the nose points (in red) between the tested fish (white) and the social stimulus (purple). (e,f) Social behavior of setd5+/+ and setd5+/− adult fish, evaluated in terms of distance between the nose points of the subjects, untreated (e) and Risperidone-treated fish (f). (b,c,e,f) Data are expressed as mean ± SEM; n = number of fish analyzed. (b,c,e,f) Statistical analysis was performed using (b,c) 1-way ANOVA followed by post-hoc test or (e,f) Student’s t-test. * p < 0.05; *** p < 0.001.
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