FIGURE

Figure 3

ID

ZDB-FIG-221230-37

Publication

Bello-Rojas et al., 2022 - Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits

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Figure 3

Sister V2a/b neurons project along similar trajectories, although V2a neurons are consistently longer.

(A) Confocal image of chx10:Red larva exhibiting a single vsx1 GFP+ clonal pair with long axons in close proximity to each other. Stitched maximum intensity projection over 74 z-planes (74 μm). (B) Scatterplot of sister V2a axon length vs. sister V2b axon length (n = 27) for V2a/b pairs. Heat map depicts the muscle segment number where each clonal pair was located. Black line depicts Pearson correlation, r = 0.32, p=0.10. V2a axons were invariably longer than sister V2b axons, as seen by each pair’s position relative to the unity line (dashed gray). ***Wilcoxon signed-rank test, p=1.9 × 10^–5, paired t-test n = 27 pairs. (C) Histogram of clonal pairs showing the fraction of V2b axon that is within 5 μm of V2a axon. Inset schematic depicts how the distances were measured. Source data for this figure are given in Figure 3—source data 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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