PUBLICATION
Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits
- Authors
- Bello-Rojas, S., Bagnall, M.W.
- ID
- ZDB-PUB-221230-4
- Date
- 2022
- Source
- eLIFE 11: (Journal)
- Registered Authors
- Bagnall, Martha, Bello-Rojas, Saul
- Keywords
- Notch, clonal relationships, development, motor circuit, motor neurons, motor systems, neuroscience, notch signaling, sister neurons, spinal cord, v2a neuron, v2b neuron
- MeSH Terms
-
- Animals
- Cell Differentiation
- Interneurons*/physiology
- Mammals
- Neurons
- Spinal Cord/physiology
- Zebrafish*/metabolism
- PubMed
- 36580075 Full text @ Elife
Citation
Bello-Rojas, S., Bagnall, M.W. (2022) Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits. eLIFE. 11:.
Abstract
Shared lineage has diverse effects on patterns of neuronal connectivity. In mammalian cortex, excitatory sister neurons assemble into shared microcircuits. In Drosophila, in contrast, sister neurons with different levels of Notch expression (NotchON/NotchOFF) develop distinct identities and diverge into separate circuits. Notch-differentiated sister neurons have been observed in vertebrate spinal cord and cerebellum, but whether they integrate into shared or distinct circuits remains unknown. Here, we evaluate how sister V2a (NotchOFF)/V2b (NotchON) neurons in the zebrafish integrate into spinal circuits. Using an in vivo labeling approach, we identified pairs of sister V2a/b neurons born from individual Vsx1+ progenitors and observed that they have somata in close proximity to each other and similar axonal trajectories. However, paired whole-cell electrophysiology and optogenetics revealed that sister V2a/b neurons receive input from distinct presynaptic sources, do not communicate with each other, and connect to largely distinct targets. These results resemble the divergent connectivity in Drosophila and represent the first evidence of Notch-differentiated circuit integration in a vertebrate system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping