FIGURE

Figure 6

ID

ZDB-FIG-221230-40

Publication

Bello-Rojas et al., 2022 - Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits

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Figure 6

Optical stimulation elicits spiking in stochastically labeled vsx1 sister neurons expressing CoChR2.

(A) Maximum intensity projection (79 planes, 79 μm) of WT larva with a single vsx1:Gal4;UAS:CoChR2-tdTomato+ clonal pair. (B) Schematic of cell-attached recording of vsx1:Gal4;UAS:CoChR2-tdTomato+ neurons using optical stimulation. (C) Cell-attached example trace of CoChR2+V2a neuron during optical stimulation (top, green) (n = 13 from 12 fish). Cell-attached example trace of nearby CoChR2- neuron during optical stimulation (bottom, black) (n = 22 from 18 fish). Both example traces are aligned to the start of the 10ms optical stimulus (light blue). (D) Averaged detected spike events recorded from an example CoChR2+ sister V2a neuron (top, red), CoChR2+ sister V2b neuron (middle, blue), and absence of response in nearby CoChR2- neuron (bottom, black). (E) Histogram showing the number of spikes relative to the optical stimulus. Blue bar indicates the duration of the optical stimulus. Source data for this figure are given in Figure 6—source data 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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