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Figure 3
(A) Confocal image of chx10:Red larva exhibiting a single vsx1 GFP+ clonal pair with long axons in close proximity to each other. Stitched maximum intensity projection over 74 z-planes (74 μm). (B) Scatterplot of sister V2a axon length vs. sister V2b axon length (n = 27) for V2a/b pairs. Heat map depicts the muscle segment number where each clonal pair was located. Black line depicts Pearson correlation, r = 0.32, p=0.10. V2a axons were invariably longer than sister V2b axons, as seen by each pair’s position relative to the unity line (dashed gray). ***Wilcoxon signed-rank test, p=1.9 × 10–5, paired t-test n = 27 pairs. (C) Histogram of clonal pairs showing the fraction of V2b axon that is within 5 μm of V2a axon. Inset schematic depicts how the distances were measured. Source data for this figure are given in Figure 3—source data 1.
Sister V2a/b neurons project along similar trajectories, although V2a neurons are consistently longer.