FIGURE

Fig. 3

ID

ZDB-FIG-221223-11

Publication

Qin et al., 2021 - Uptake of oxidative stress-mediated extracellular vesicles by vascular endothelial cells under low magnitude shear stress

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Low magnitude shear stress promotes the uptake of RBCEVs by endothelial cells. (a) Model diagram of orbital shaker system. (b) Laser confocal images of RBCEVs that were uptake by endothelial cells at different shear stresses for 3 h. (c) and (g) Flow cytometry analysis of the uptake of RBCEVs by endothelial cells for 3 h. (d) Mechanical loading equipment model diagram of the parallel-plate flow chamber. (h), (j), and (l) Laser confocal images of RBCEVs that were uptake by endothelial cells under NSS, LSS, and OSS for 3 h. (i), (k), and (m) Imaris 3D rendering for (h), (j), and (l), respectively. (n), (o), and (p) XYZ axis showed uptake in different directions. (e) Quantitation of the number of RBCEVs uptake by endothelial cells and (f) mean fluorescence intensity of RBCEVs from (h), (j), and (l). (n = 5) (scale bar = 20 μm). Significance is indicated as no significance (ns), or P < 0.001 (***).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Bioact Mater