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Fig. 3
Low magnitude shear stress promotes the uptake of RBCEVs by endothelial cells. (a) Model diagram of orbital shaker system. (b) Laser confocal images of RBCEVs that were uptake by endothelial cells at different shear stresses for 3 h. (c) and (g) Flow cytometry analysis of the uptake of RBCEVs by endothelial cells for 3 h. (d) Mechanical loading equipment model diagram of the parallel-plate flow chamber. (h), (j), and (l) Laser confocal images of RBCEVs that were uptake by endothelial cells under NSS, LSS, and OSS for 3 h. (i), (k), and (m) Imaris 3D rendering for (h), (j), and (l), respectively. (n), (o), and (p) XYZ axis showed uptake in different directions. (e) Quantitation of the number of RBCEVs uptake by endothelial cells and (f) mean fluorescence intensity of RBCEVs from (h), (j), and (l). (n = 5) (scale bar = 20 μm). Significance is indicated as no significance (ns), or P < 0.001 (***).