Fig. 3

Comparison between 3D immunostaining of tissue cleared with SDS and SDS‐OPTIClear. (A) Iba‐1 staining of tissues cleared with 4% SDS‐OPTIClear (left panel) and 4% SDS only (right panel). Upper panel: images taken with a 10× objective. Lower panel: zoomed‐in image of the dentate gyrus (DG) taken with a 20× objective (left image), Z‐stack images taken with a 63× objective with z‐depth = 161.7 μm (right image). (B) TOMM‐20 staining of regions cleared with 4% SDS‐OPTIClear (left panel) and 4% SDS only (right panel). Left image: 20× images of DG regions. Right image: z‐stack of the images showed on the left (z‐depth = 125.84 μm). (C) ChAT staining taken with a 20× objective. Left panel: 20× images of the nbM cleared with 4% SDS and 4% SDS‐OPTIClear. Right panel: z‐stack of the images showed in the left panel (z‐depth = 72 μm). The same imaging and display settings were used for both 4% SDS and 4% SDS with OPTIClear conditions