Fig. 1

Tissue clearing with different concentrations of SDS vs SDS?OPTIClear. (A) Representative images of 4 mm?thick rat brain tissues before and after clearing with SDS and SDS?OPTIClear respectively. Scale bar = 5 mm. (B) Absorbance at 750 nm for tissues cleared at varying concentrations of SDS vs SDS?OPTIClear (n = 5?6). Lower absorbance level indicates higher transparency level. (C) Fold of area change after clearing with SDS compared to SDS?OPTIClear (n = 3). Tissue sizes were quantified and normalised to the size of tissue before clearing. (D) Fold of protein loss after clearing for SDS only compared to SDS?OPTIClear (n = 5?6). Solutions used for clearing were collected and then added to a stain?free gel for electrophoresis, band intensities were quantified and normalised with the protein band intensity of sample solution collected at day 0 (before clearing). Mean ± SEM for all graphs. Two?way ANOVA, Tukey's multiple comparison test, *p ? 0.05, **p ? 0.01, ***p ? 0.001, ****p ? 0.0001