FIGURE

Fig 3

ID

ZDB-FIG-221216-42

Publication

Grzegorski et al., 2022 - Genetic duplication of tissue factor reveals subfunctionalization in venous and arterial hemostasis

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Fig 3

Vascular development is unaffected in the absence of TF.

(A) Example of a 30 hpf larva carrying the kdrl:eGFP transgene marking vascular endothelial cells (top) with a close-up (below). White arrowheads mark examples of vessel sprouts that have made it at least halfway through the expected migration. Orange arrowheads represent sprouts that have not yet passed the halfway point. No statistically significant changes were observed between groups in total vessel sprouts (B) or sprouts that have made it past the halfway point (C) by Mann-Whitney U testing. (D) At 54 hpf, no observable changes are seen in endothelial cell development (left, green) or in vascular integrity (right, red). The latter is evidenced by long exposure in the gata1:DsRed transgenic background in which erythrocytes are marked by red fluorescence. Representative images for 14 experimental mutant larvae that were scored by a blinded observer are shown along with a wild type control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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