FIGURE

Fig. 3

ID

ZDB-FIG-221214-14

Publication

Zaupa et al., 2021 - Trans-inhibition of axon terminals underlies competition in the habenulo-interpeduncular pathway

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Nicotine induces prolonged negatively correlated event

(A) Representative ΔF/F traces from dHb terminals in Tg(gng8:GAL4ff)^c426;Tg(UAS:GCaMP7a) explant. 5 min nicotine (10 μM) perfusion induces inhibition at the dIPN (top) and bursts at the raphe (bottom).

(A’) Average ΔF/F traces (black) of nicotine-induced negatively correlated events (grey). Duration (cyan boxes) and initiation (red dotted lines) of nicotine application indicated. n = 8 explants.

(B,C) Representative raster plots of dIPN inhibition and raphe burst events. (B) Control and (C) nAChR antagonist (100 μM mecamylamine and 10 μM d-tubocurarine) application. Introduction of E3 solution (B) or antagonists (C) indicated (red lines).

(B’,C’) Ratio of average number of events in 15-minute windows (I, II, III) and baseline activity (b) after applying (B’) E3 (n = 4) or (C’) antagonists (n = 6). Bars indicate negatively correlated events in dHb terminals at the raphe (green, bursts) and dIPN (red, inhibition). One-way ANOVA followed by Tukey’s test, ** p<0.005. b: 15 minutes of baseline, I: 0–15, II: 15–30, III: 30–45 min after application.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.