ZFIN Image: Zaupa et al., 2021, Fig. 3

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Figure Caption

Fig. 3

Nicotine induces prolonged negatively correlated event

(A) Representative ?F/F traces from dHb terminals in Tg(gng8:GAL4ff)^c426;Tg(UAS:GCaMP7a) explant. 5 min nicotine (10 ?M) perfusion induces inhibition at the dIPN (top) and bursts at the raphe (bottom).

(A?) Average ?F/F traces (black) of nicotine-induced negatively correlated events (grey). Duration (cyan boxes) and initiation (red dotted lines) of nicotine application indicated. n = 8 explants.

(B,C) Representative raster plots of dIPN inhibition and raphe burst events. (B) Control and (C) nAChR antagonist (100 ?M mecamylamine and 10 ?M d-tubocurarine) application. Introduction of E3 solution (B) or antagonists (C) indicated (red lines).

(B?,C?) Ratio of average number of events in 15-minute windows (I, II, III) and baseline activity (b) after applying (B?) E3 (n = 4) or (C?) antagonists (n = 6). Bars indicate negatively correlated events in dHb terminals at the raphe (green, bursts) and dIPN (red, inhibition). One-way ANOVA followed by Tukey?s test, ** p<0.005. b: 15 minutes of baseline, I: 0?15, II: 15?30, III: 30?45 min after application.

Acknowledgments

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