FIGURE

Fig. 2

ID

ZDB-FIG-221127-43

Publication

Cudia et al., 2021 - NMR and EPR-DEER Structure of a Dimeric Guanylate Cyclase Activator Protein-5 from Zebrafish Photoreceptors

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Fig. 2

Residual dipolar coupling (RDC) structural analysis of GCAP5. ¹H?¹⁵N IPAP-HSQC spectra of Mg²⁺-bound GCAP5 in the (A) absence and (B) presence of 12 mg/mL Pf1 phage. Observed spectral splittings in the absence of Pf1 (J_NH) and presence of Pf1 (J_NH + D_NH) are marked by vertical lines, and their difference was used to calculate RDCs as described in Materials and Methods. (C) RDCs calculated from the structure of Mg²⁺-bound GCAP5 in Figure 3 plotted vs the RDCs measured in panel B show good agreement (Q-factor = 0.27, and R-factor = 0.985³²).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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