FIGURE

Fig. 4

ID

ZDB-FIG-221127-45

Publication

Cudia et al., 2021 - NMR and EPR-DEER Structure of a Dimeric Guanylate Cyclase Activator Protein-5 from Zebrafish Photoreceptors

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

EPR-DEER intermolecular distances for the GCAP5 dimer. Representative EPR-DEER data of (A) GCAP5^CL(A28C), (C) GCAP5^CL(N56C), and (E) GCAP5^CL(A69C) and corresponding distance distributions of (B) GCAP5^CL(A28C), (D) GCAP5^cl(N56C), and (F) GCAP5^CL(A69C). GCAP5 samples were in the Ca²⁺-free, Mg²⁺-bound state. Similar DEER data were observed for GCAP5 in the Ca²⁺-bound state (not shown). A nitroxide spin-label (MTSSL) was covalently attached to the sole Cys residue in each mutant. The distance distributions and average intermolecular distances were calculated on the basis of the DEER data as described in Materials and Methods. The DEER intermolecular distances were measured to be 16 ± 1 Å [GCAP5^CL(A28C) in panel B], 52 ± 3 Å [GCAP5^CL(N56C) in panel D], and 41 ± 3 Å [GCAP5^CL(A69C) in panel F].

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochemistry