FIGURE

Figure 4.

ID

ZDB-FIG-221121-29

Publication

Jeon et al., 2022 - Ohmyungsamycin Promotes M1-like Inflammatory Responses to Enhance Host Defense against Mycobacteroides abscessus Infections

Other Figures

All Figure Page

Back to All Figure Page

Figure 4.

OMS treatment induces an M1-like state in macrophages during Mabc infection.(a, b) qPCR analysis of Tnf, Il1b, Il12p40 (a), and Ccl5 (b) mRNA expression in BMDMs. BMDMs were infected with Mabc (MOI of 3), with or without OMS treatment (5 μM), for the indicated times. (c) ELISA of TNF in the supernatant from PMs. PMs were infected with Mabc (MOI of 1), with or without OMS treatment (5 μM) for the indicated times. (d, e) Flow cytometry of CD86 expression in BMDMs. BMDMs were infected with Mabc (MOI of 1), with or without OMS treatment (5 μM) for 3 days. (d) Mean fluorescence intensity of CD86. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparison test (a – c, d). Data are means ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. BMDMs, bone marrow derived macrophages, a.u., arbitrary unit.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Virulence