Figure 5.

NF-κB signalling pathway activation contributes to OMS-induced antimicrobial responses in BMDMs during Mabc infection. (a) Representative immunoblots in BMDMs. BMDMs were infected with Mabc (MOI of 1), with or without OMS treatment (5 μM) for the indicated times and probed with antibody to phospho (p)-p65, p-ERK1/2 (p-ERK), p-JNK1/2 (p-JNK), p-p38 MAPK (p-p38) or actin. (b) Densitometric quantifications of p-p65. (c) Intracellular survival assay in BMDMs. BMDMs were pre-incubated for 1 h with or without indicated concentration (1 or 5 μM) of either BAY or TPCA-1, followed by infection with Mabc (MOI of 1). Cells were then treated with OMS (5 μM) in the presence or absence of either BAY or TPCA-1 (1 or 5 μM) for 2 days. Statistical significance was calculated using unpaired student t-test (for b) and One-way ANOVA with Tukey’s multiple comparison test (for c). Data are presented as mean ±SD. OMS, Ohmyungsamycin A; BAY, BAY 11–7082; CFU, colony forming units; n.s., not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.