FIGURE

Fig. 3

ID

ZDB-FIG-221119-22

Publication

Wei et al., 2022 - Anti-infective therapy using species-specific activators of Staphylococcus aureus ClpP

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Fig. 3

Effect of (R)- (a) and (S)-ZG197 (b) on the thermal stability of SaClpP and HsClpP in DSF assay, respectively. T_m, melting temperature. c Determination of (R)- and (S)-ZG197 binding to SaClpP by ITC titration. The dissociation constant (K_d) and stoichiometry factor (N) are indicated. Effect of activators on the thermal stability of the cellular SaClpP (d) and HsClpP (e), respectively. The S. aureus 8325-4 was cultured in the presence of 10 μM compound for 2 h before running CETSA (d), while cell lysates of HEK 293 T/17 were assayed in CETSA (e). The proteins in unheated samples are used as inputs and considered as 100%. Data (a, b, d, and e) are obtained from three biologically independent experiments and presented as mean ± SD (error bars). Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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