FIGURE

Fig. 3

ID

ZDB-FIG-221109-32

Publication

Zhou et al., 2022 - Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction

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Fig. 3

a, b Intracellular staining, flow cytometry and corresponding quantitative analysis of DUSP6 levels in various cell types in wild-type LV tissue at 72 h after MI. n = 5 (CMs, SMCs & Fibroblasts) or 6 (VECs, Neutrophils, Macrophages) biological independent samples/group. Gating strategies for each cell population are shown in Supplementary Fig. 4a. c Representative immunohistochemistry of DUSP6 (dark brown) in wild-type LV tissue at sham operation, 24 h, 72 h, and 7 days after MI (n = 9 areas from 3 hearts/group; scale bar, 100 μm). d–f Representative double immunofluorescence of DUSP6 with either HIS48, MPO, or PR3 in wild-type LV tissue at 72 h after MI, showing DUSP6 expression in neutrophils (n = 9 areas from 3 hearts/group; scale bar, 10 μm). DAPI co-staining was used to display nuclear morphology. All quantitative data shown in this figure are presented as mean values ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to calculate the presented p-values. Source data of b are provided in a Source Data File. CMs cardiomyocytes, VECs vascular endothelial cells, SMCs smooth muscle cells, MPO myeloperoxidase, PR3 proteinase 3, MFI median fluorescence intensity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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