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Fig. 3 a, b Intracellular staining, flow cytometry and corresponding quantitative analysis of DUSP6 levels in various cell types in wild-type LV tissue at 72 h after MI. n = 5 (CMs, SMCs & Fibroblasts) or 6 (VECs, Neutrophils, Macrophages) biological independent samples/group. Gating strategies for each cell population are shown in Supplementary Fig. 4a. c Representative immunohistochemistry of DUSP6 (dark brown) in wild-type LV tissue at sham operation, 24 h, 72 h, and 7 days after MI (n = 9 areas from 3 hearts/group; scale bar, 100 μm). d–f Representative double immunofluorescence of DUSP6 with either HIS48, MPO, or PR3 in wild-type LV tissue at 72 h after MI, showing DUSP6 expression in neutrophils (n = 9 areas from 3 hearts/group; scale bar, 10 μm). DAPI co-staining was used to display nuclear morphology. All quantitative data shown in this figure are presented as mean values ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to calculate the presented p-values. Source data of b are provided in a Source Data File. CMs cardiomyocytes, VECs vascular endothelial cells, SMCs smooth muscle cells, MPO myeloperoxidase, PR3 proteinase 3, MFI median fluorescence intensity.