FIGURE

Fig. 5

ID

ZDB-FIG-221107-5

Publication

Choo et al., 2021 - Identification of curcumin analogues with anti-seizure potential in vivo using chemical and genetic zebrafish larva seizure models

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Fig. 5

Fig. 5. CA 80(1) and CA 20(1) are preventing brain seizures in gabrg2-/- larvae. (A) Timeline of the experimental procedure corresponding to our brain activity assay using gabra1-/- and gabrg2-/- genetic seizure models that have been crossed with the [neurod:GCaMP6f] transgenic line [1]. (B, C) Quantification of the fluorescence intensity upon LASER excitation (averaged normalised mean grey value for 2 s) of gabra1 and gabrg2 5 dpf larvae that have been pre-incubated with vehicle (VHC) or analogues at 2–5 µM. The * ** * marker denotes significance at P < 0.0001 between the indicated groups using two-way Analysis of Variance (ANOVA). (D & E) Single confocal images corresponding to the first 400 ms after LASER light exposure showing neuronal activity of gabra1 and gabrg2 mutants pre-incubated with vehicle (VHC) or specific analogues.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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