Fig. 5

Fig. 5

Fig. 5. CA 80(1) and CA 20(1) are preventing brain seizures in gabrg2-/- larvae. (A) Timeline of the experimental procedure corresponding to our brain activity assay using gabra1-/- and gabrg2-/- genetic seizure models that have been crossed with the [neurod:GCaMP6f] transgenic line [1]. (B, C) Quantification of the fluorescence intensity upon LASER excitation (averaged normalised mean grey value for 2 s) of gabra1 and gabrg2 5 dpf larvae that have been pre-incubated with vehicle (VHC) or analogues at 2–5 µM. The * ** * marker denotes significance at P < 0.0001 between the indicated groups using two-way Analysis of Variance (ANOVA). (D & E) Single confocal images corresponding to the first 400 ms after LASER light exposure showing neuronal activity of gabra1 and gabrg2 mutants pre-incubated with vehicle (VHC) or specific analogues.