Fig. 5

Histological analysis of ocular anomalies in foxc1a^?CED1?3 homozygous embryos. A, A? H&E-stained transverse sections of the eye of 6-dpf wild-type and mutant embryos. B?C? H&E-stained transverse sections through central (B and B?) and nasal (C and C?) eye regions of 30-dpf wild-type and mutant fish. Mutants show a marked enlargement of the anterior chamber and abnormal development of both dorsal and ventral annular ligaments (orange arrows, A??C?); dislocation of lenses toward the back of the eye (B?); and deformed/misplaced scleral ossicles at the dorsal irido-corneal angle (black arrowhead, B?). D?E? 20× magnifications of the dorsal irido-corneal angle (D and D?) and cornea (E and E?) showing details of the hypoplastic dorsal annular ligament (orange arrow, D?), and thin cornea at 30-dpf (orange arrowhead, E?). Transverse (F and F?) and coronal (G and G?) 40× magnifications of the ventral irido-corneal angle and canalicular network showing an apparent absence of the glycoprotein aggregates in the ventral annular ligament (orange arrow in F?), narrowing of the irido-corneal canal (blue arrow, F?), hyperplasia of the ventral iris stroma in this region (orange asterisks in F? and G?) and thin cornea at 30-dpf (orange arrowhead in F?). H?K? immunostaining of cornea sections of 30-dpf wild-type and mutant fish with anti-CKS (red) and anti-cdh2 (green), showing a thinner corneal stroma (I?) and a disorganized corneal epithelium (J?). AL, annular ligament; C, cornea; CaN, canalicular network; CC, ciliary canal; ce, corneal epithelium; cn, corneal endothelium; cs, corneal stroma; I, iris; IC, irido-corneal canal; Le, lens; ON, optic nerve; Os, scleral ossicle R, retina