FIGURE

Figure 2.

ID

ZDB-FIG-221030-18

Publication

Wang et al., 2022 - Hoxa9/meis1-transgenic zebrafish develops acute myeloid leukaemia-like disease with rapid onset and high penetrance

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Figure 2.

Transgenic zebrafish with inducible expression of meis1 under the control of hsp70 promoter. (a) The schematic representation of Tg(hsp70:meis1) expression vector. Ubiquitous GFP expression can be observed throughout whole embryos followed by HS for 1 h at 24 hpf (white arrowheads). (b) Western blot using a monoclonal anti-Meis1 antibody recognizes Meis1 protein at different times after HS induction. The antibody also recognizes endogenous Meis1 protein in the control group without HS. The mouse anti-β-actin antibody serves as controls. (c) Tg(hsp70:meis1) embryos were heat shocked at 24 hpf and assayed by WISH for runx1 (2 dpf), c-myb (3 dpf), mpx (3 dpf) and mfap4 (5 dpf) at different stages. (d) The quantification of WISH results in C (n = 50–100 embryos per group). Data are represented as mean ± s.d. NS means no statistical significance. (e) Benzidine staining of haemoglobin in mature erythrocytes in embryos at 4–5 dpf. HS, heat-shock. dpf, days post-fertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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