ZFIN Image: Wang et al., 2022, Figure 2<tag0:x xml:space='preserve'>. </tag0:x>

Image

Figure Caption

Figure 2.

Transgenic zebrafish with inducible expression of meis1 under the control of hsp70 promoter. (a) The schematic representation of Tg(hsp70:meis1) expression vector. Ubiquitous GFP expression can be observed throughout whole embryos followed by HS for 1 h at 24 hpf (white arrowheads). (b) Western blot using a monoclonal anti-Meis1 antibody recognizes Meis1 protein at different times after HS induction. The antibody also recognizes endogenous Meis1 protein in the control group without HS. The mouse anti-β-actin antibody serves as controls. (c) Tg(hsp70:meis1) embryos were heat shocked at 24 hpf and assayed by WISH for runx1 (2 dpf), c-myb (3 dpf), mpx (3 dpf) and mfap4 (5 dpf) at different stages. (d) The quantification of WISH results in C (n = 50–100 embryos per group). Data are represented as mean ± s.d. NS means no statistical significance. (e) Benzidine staining of haemoglobin in mature erythrocytes in embryos at 4–5 dpf. HS, heat-shock. dpf, days post-fertilization.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Open Biol.