FIGURE

Fig. 3

ID

ZDB-FIG-221026-65

Publication

Naylor et al., 2022 - Basement membrane defects in CD151-associated glomerular disease

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Immunofluorescence images of CD151 and Col4α3 in normal human kidney glomeruli and a patient with loss of function variant in the CD151 gene. IF images left to right: co-IF (CD151 green; Col4α3 red), CD151 and Col4α3. Scale bars = 50 µm, insert scale bar = 10 µm. A) Representative glomeruli from normal human adult control; B) representative glomeruli from patient kidney biopsy – fluorescence was significantly less than control and was adjusted using Fiji/ImageJ to make easily visible; C) quantification of CD151 expression in control and patient glomeruli; D) quantification of Col4α3 expression in control and patient glomeruli. Mean grey values were obtained for the region of the capillary tuft using Fiji/ImageJ and the mean ± SD and an unpaired t-test (p < 0.0001) was performed using GraphPad Prism. Control glomeruli n = 26; Patient glomeruli n = 17

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Pediatr. Nephrol.