Fig. 3

Fig. 3

Immunofluorescence images of CD151 and Col4α3 in normal human kidney glomeruli and a patient with loss of function variant in the CD151 gene. IF images left to right: co-IF (CD151 green; Col4α3 red), CD151 and Col4α3. Scale bars = 50 µm, insert scale bar = 10 µm. A) Representative glomeruli from normal human adult control; B) representative glomeruli from patient kidney biopsy – fluorescence was significantly less than control and was adjusted using Fiji/ImageJ to make easily visible; C) quantification of CD151 expression in control and patient glomeruli; D) quantification of Col4α3 expression in control and patient glomeruli. Mean grey values were obtained for the region of the capillary tuft using Fiji/ImageJ and the mean ± SD and an unpaired t-test (p < 0.0001) was performed using GraphPad Prism. Control glomeruli n = 26; Patient glomeruli n  = 17