Fig. 5

Fig. 5. Zebrafish sensory neurons maintain a somatic hotspot of microtubule organization. A. Brightfield image of a 3rd instar drosophila larva and image of a ddaE neuron cell body expressing EB1-GFP and fzr-RFP are shown. The blue line indicates the region used to quantify EB1-GFP intensity from a sum projection of a timeseries. The output for fzr-RFP (red) and EB1-GFP (green) is shown in the graph. B. A brightfield image of a 4?6 tentacle stage Nematostella vectensis polyp and an image of a tripolar ganglion cell expressing EB1-GFP are shown. The blue line was used as the region for summing intensity through the time course and the output is shown in the graph. C. A brightfield image of a 14dpf zebrafish and images of DRG neuron cell bodies expressing EB3-GFP are shown together with lines used to quantify EB3-GFP fluorescence. The graphs show intensity along the line summed through the timecourse. D.-G. Brightfield overviews and images of RB neuron cell bodies expressing EB3-GFP are shown from different age fish. In each a line was used to generate summed intensity graphs.