PUBLICATION
Microtubule organization of vertebrate sensory neurons in vivo
- Authors
- Shorey, M., Rao, K., Stone, M.C., Mattie, F.J., Sagasti, A., Rolls, M.M.
- ID
- ZDB-PUB-210622-34
- Date
- 2021
- Source
- Developmental Biology 478: 1-12 (Journal)
- Registered Authors
- Rolls, Melissa, Sagasti, Alvaro, Shorey, Matthew, Stone, Michelle
- Keywords
- Dorsal root ganglion cell, Microtubule polarity, Neuronal polarity
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/physiology
- Axons/ultrastructure
- Cell Body/ultrastructure
- Cell Polarity
- Dendrites/physiology
- Drosophila/cytology
- Drosophila/growth & development
- Ganglia, Spinal/physiology
- Ganglia, Spinal/ultrastructure*
- Microtubule-Organizing Center/ultrastructure
- Microtubules/ultrastructure*
- Sea Anemones/cytology
- Sea Anemones/growth & development
- Sea Anemones/ultrastructure
- Sensory Receptor Cells/physiology
- Sensory Receptor Cells/ultrastructure*
- Skin/innervation*
- Zebrafish
- PubMed
- 34147472 Full text @ Dev. Biol.
Citation
Shorey, M., Rao, K., Stone, M.C., Mattie, F.J., Sagasti, A., Rolls, M.M. (2021) Microtubule organization of vertebrate sensory neurons in vivo. Developmental Biology. 478:1-12.
Abstract
Dorsal root ganglion (DRG) neurons are the predominant cell type that innervates the vertebrate skin. They are typically described as pseudounipolar cells that have central and peripheral axons branching from a single root exiting the cell body. The peripheral axon travels within a nerve to the skin, where free sensory endings can emerge and branch into an arbor that receives and integrates information. In some immature vertebrates, DRG neurons are preceded by Rohon-Beard (RB) neurons. While the sensory endings of RB and DRG neurons function like dendrites, we use live imaging in zebrafish to show that they have axonal plus-end-out microtubule polarity at all stages of maturity. Moreover, we show both cell types have central and peripheral axons with plus-end-out polarity. Surprisingly, in DRG neurons these emerge separately from the cell body, and most cells never acquire the signature pseudounipolar morphology. Like another recently characterized cell type that has multiple plus-end-out neurites, ganglion cells in Nematostella, RB and DRG neurons maintain a somatic microtubule organizing center even when mature. In summary, we characterize key cellular and subcellular features of vertebrate sensory neurons as a foundation for understanding their function and maintenance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping