FIGURE

FIGURE 2

ID

ZDB-FIG-220921-60

Publication

Field et al., 2022 - Involvement of transmembrane protein 184a during angiogenesis in zebrafish embryos

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FIGURE 2

Tmem184a KD Results in Decreased Number of Intact Intersegmental Vessels. (A,A′) SC MO injected embryo (n = 35). (B,B′) Tmem184a SB MO injected embryo (n = 25). (C,C′) Tmem184a ATG MO injected embryo (n = 19). Asterisks indicate ISVs that failed to complete injected embryos. (D,D′) Injection with full-length Tmem184a mRNA alone (n = 21). (E,E′) Co-injection with full-length Tmem184a mRNA rescues vascular defects in Tmem184a SB MO injected embryos (n = 15). (A′–E′) illustrate zoomed trunk regions of the fish. (F). KD by SB and ATG MOs compared to SC MO embryos and rescue embryos with SB MO and Tmem184a mRNA, illustrating the mean number of intact ISVs ± SEM. p < 0.001. (G) Embryo survival for all treatments shown as compared to SC embryo survival at 24 h ± SEM p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO1-tmem184a y1Tg + MO2-tmem184a
Knockdown Reagents:	MO1-tmem184a MO2-tmem184a
Observed In:	dorsal longitudinal anastomotic vessel intersegmental vessel
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.