The effect of ring1b deficiency on the early development of brain in zebrafish embryos. (A) The expression of pax2a in rnf2–/– and WT embryos at 12 hpf. Upper: lateral view, bottom: dorsal view. The red and white arrows, respectively, showing the different expression of pax2a in WT and rnf2–/– embryos at 12 hpf. (B) The expression of egr2b in rnf2–/– and WT embryos at 12 hpf. Upper: lateral view, bottom: dorsal view. The red arrows showing the different expression of egr2b in WT and rnf2–/– embryos at 12 hpf. (C) The expression of cyp26c1 in rnf2–/– and WT embryos at 12 hpf. Upper: lateral view, bottom: dorsal view. The red arrows showing the different expression of cyp26c1 in WT and rnf2–/– embryos at 12 hpf. (D) The expression of ngn1 in rnf2–/– and WT embryos at 12 hpf. The experiments were repeated at least three times. Scale bar = 0.1 mm.
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