FIGURE

Fig. 2

ID

ZDB-FIG-220921-124

Publication

Andreassen et al., 2022 - Brain dysfunction during warming is linked to oxygen limitation in larval zebrafish

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Fig. 2

Embedded zebrafish larvae develop brain-wide depolarizations near the upper thermal limit. (A and B) Schematic overview (A) and image (B) of the experimental setup for whole-brain neural activity measurement in agar-embedded, 5-d-old Tg(elavl3:GCaMP6s) zebrafish larva. (B) Raw fluorescence image of the larva brain highlighting the telencephalon (dashed green lines) and medulla (dashed magenta lines). A, anterior; L, lateral. (C and D) Change in fluorescence (% ?F/F₀; left y-axis) in the telencephalon and medulla of a representative control larva (C) and a representative heat-ramp larva (D). The water temperature (gray) was maintained at 28 �C throughout the recording (right y-axis in C) for the control fish and was increased during a heat-ramp treatment (0.3 �C/min) until a brain-wide depolarization was detected and then rapidly adjusted to 28 �C until the end of the recording (right y-axis in D). Note the return to holding temperature in heat-ramp fish and recovery of normal brain activity and calcium peaks 10 to 15 min after the brain-wide depolarization in D. (E) Heat map illustrating the temporal spread of the depolarization throughout the brain in a representative heat-ramp fish mounted laterally (Methods). D, dorsal. (F) Frequency of medulla calcium peaks in heat-ramp fish (n = 11; magenta line) during the 50 min preceding the brain-wide depolarization and during the corresponding period in control fish (n = 8; magenta dashed line) (SI Appendix, Table S4). The depolarization onset in heat-ramp fish is indicated by a dashed vertical line. (G) Temperature at depolarization onset in heat-ramp fish (n = 11). (F and G) Data are presented with mean (solid line) and SE (shaded area) in (F) and with a bar and error bars in (G).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA