The upper thermal limit in freely swimming zebrafish larvae and adults. (A and B) Schematic figure (A) and photo with top view (B) of the experimental setup for CTmax measurement in freely swimming zebrafish larvae. (B) Larva initial position is indicated by a yellow, filled arrowhead and the trajectory is represented in yellow during the following 30 s. The dashed black circle indicates the walls of the central chamber. The white arrowhead indicates the position of a thermocouple. The white asterisk indicates the tube for bubbling. (C) CTmax (temperature at loss of response for 5 and 9 d postfertilization [dpf] and loss of equilibrium for adults; see Methods) in 5-d-old (n = 12; black circles), 9-d-old (n = 12; dark gray circles), and adult fish (n = 15; light gray circles) (SI Appendix, Table S3). The bars and error bars indicate the group mean ± SE.
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