FIGURE

Fig. 4

ID

ZDB-FIG-220819-38

Publication

Morales et al., 2022 - Interleukin-10 regulates goblet cell numbers through Notch signaling in the developing zebrafish intestine

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Fig. 4

Increased alcian blue⁺ goblet cells in il10 mutant larvae are independent of the microbiota.

a Diagram showing bleach (sodium hypochlorite 0.004%) plus antibiotic (Abx) treatment in zebrafish embryos/larvae (Abx: Ampicillin 100 µg/mL, Kanamycin 5 µg/mL). b Quantification of bacterial 16S DNA levels over zebrafish genomic DNA measured by PCR. Each dot corresponds to a pool of 2 larvae from 4 independent experiments. c Transcriptomic expression analysis of cytokines from treated larvae by qRT-PCR. Each dot represents a pool of 8–10 larvae collected from 4 independent experiments (d, e) Alcian blue staining and analysis on 5dpf WT and il10-Mut larvae treated with Bleach+Abx and controls (N = 2 independent experiments). Scale bar = 100 µm. Independent experiments are shown with different symbol shapes in b, c and e. Two-way ANOVAs with Fisher’s LSD multiple comparisons tests were performed in b, c and e (*p < 0.05; **p < 0.01; ***p < 0.001).

Expression Data

Genes:	ifng1 il17a/f3
Fish:	il10^{uu1751/uu1751}
Condition:	chemical treatment by environment: sodium hypochlorite
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB il10^{uu1751/uu1751}
Condition:	chemical treatment by environment: sodium hypochlorite
Observed In:	mid intestine mid intestine goblet cell mid intestine regulation of intestinal epithelial cell development whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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