ZFIN Image: Morales et al., 2022, Fig. 4

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Fig. 4

Increased alcian blue⁺ goblet cells in il10 mutant larvae are independent of the microbiota.

a Diagram showing bleach (sodium hypochlorite 0.004%) plus antibiotic (Abx) treatment in zebrafish embryos/larvae (Abx: Ampicillin 100 µg/mL, Kanamycin 5 µg/mL). b Quantification of bacterial 16S DNA levels over zebrafish genomic DNA measured by PCR. Each dot corresponds to a pool of 2 larvae from 4 independent experiments. c Transcriptomic expression analysis of cytokines from treated larvae by qRT-PCR. Each dot represents a pool of 8–10 larvae collected from 4 independent experiments (d, e) Alcian blue staining and analysis on 5dpf WT and il10-Mut larvae treated with Bleach+Abx and controls (N = 2 independent experiments). Scale bar = 100 µm. Independent experiments are shown with different symbol shapes in b, c and e. Two-way ANOVAs with Fisher’s LSD multiple comparisons tests were performed in b, c and e (*p < 0.05; **p < 0.01; ***p < 0.001).

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Acknowledgments

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