FIGURE

Fig. 4

ID

ZDB-FIG-220812-27

Publication

Frisk et al., 2022 - Differential expression of enzymes in thymidylate biosynthesis in zebrafish at different developmental stages: implications for dtymk mutation-caused neurodegenerative disorders

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Fig. 4

Characterization of thymidine kinases. TK activity in the cytosol (A) and nucleus (B) fractions at different zebrafish developmental stages. C DEAE chromatography of cytosolic protein isolated from zebrafish at 120 hpf. Fractions were collected and TK activity was determined by using [³ H]-dThd as substrate. D Western blot analysis of the TK peak fractions (2, 3, 4, 8, 14,15 and 16) from DEAE chromatography using a TK1 specific antibody and only part of the image is shown. Cell extracts from a fibroblast (BJ-N) cell line was used as control. E Fractions from DEAE chromatography (fraction 15 and 16) were assayed in the absence and presence of excess dCyd using [³ H]-dThd as the substrate. Data was plotted as mean?�?SD

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.