FIGURE

Fig. 3

ID

ZDB-FIG-220812-26

Publication

Frisk et al., 2022 - Differential expression of enzymes in thymidylate biosynthesis in zebrafish at different developmental stages: implications for dtymk mutation-caused neurodegenerative disorders

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Fig. 3

Characterization of cytosolic TMPK. A Partial purification of zebrafish TMPK by DEAE chromatography using cytosolic proteins isolated from zebrafish embryos harvested at 24 hpf and 120 hpf. The bound proteins were eluted with stepwise increase of KCl concentration (from 50 to 250?mM) in DEAE buffer. Fractions were collected and assayed for TMPK activity; B Protein concentration of the corresponding DEAE fractions; C Identification of the canonical TMPK proteins in the TMPK activity peak fractions by western blot analysis using a zebrafish Dtymk-specific antibody. Fractions F16, F18, F20, F21, F25, and F28 from DEAE chromatography were used and only part of the image is shown; D Size exclusion chromatography of partially purified zebrafish TMPK. Fractions F20 and F21 from the DEAE chromatography were used. Fractions were collected and assayed for TMPK activity. The Y-axis on the left side corresponded the TMPK activities from 120 hpf embryos and the Y-axis on the right side corresponded the TMPK activities from 24 hpf embryos. The elution positions of molecular weight markers are indicated

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.