ZFIN Image: Frisk et al., 2022, Fig. 4

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Figure Caption

Fig. 4

Characterization of thymidine kinases. TK activity in the cytosol (A) and nucleus (B) fractions at different zebrafish developmental stages. C DEAE chromatography of cytosolic protein isolated from zebrafish at 120 hpf. Fractions were collected and TK activity was determined by using [³ H]-dThd as substrate. D Western blot analysis of the TK peak fractions (2, 3, 4, 8, 14,15 and 16) from DEAE chromatography using a TK1 specific antibody and only part of the image is shown. Cell extracts from a fibroblast (BJ-N) cell line was used as control. E Fractions from DEAE chromatography (fraction 15 and 16) were assayed in the absence and presence of excess dCyd using [³ H]-dThd as the substrate. Data was plotted as mean?�?SD

Acknowledgments

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