Fig. 5

Optimized endogenous variant rescues loss-of-function phenotypes. (A) Schematic representation of the rescue experiment in zebrafish embryos. Variants of the slc452 gene (melanin pigmentation) were injected into loss-of-function slc45a2 knockout zebrafish embryos (albino phenotype, lack of pigmentation). The predicted mRNA stability of the variants is indicated. Illustrations by Mark Miller. (B) Quantification of the absorbance of the pigmentation as a measure of the amount of phenotype rescue. p values and replicates (n) are indicated. (C) Microscopy images of zebrafish embryos showing a degree of loss-of-pigmentation phenotype rescue by codon optimized variants compared to the albino fish. Wildtype embryo is shown in the left. Scale bars represent 700 µm. The numbers reflect the proportion of embryos that showed melanin pigmentation.