FIGURE

Figure 2.

ID

ZDB-FIG-220717-65

Publication

Mahony et al., 2021 - Hapln1b, a central organizer of the extracellular matrix, modulates kit signalling to control developmental haematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Figure 2.

Hapln1b is necessary for the specification of HSPC from the hemogenic endothelium. (A) Gata2b, runx1, and cmyb ISH in control MO, or hapln1b MO-injected embryos (injected at 3 ng throughout, unless otherwise stated). (B) ISH expression of cmyb, in control MO–, or hapln1b MO–injected embryos. (C) Rag1 expression in the thymus after control MO or hapln1b MO injection. (D) ISH expression of kdrl in control MO– or hapln1b MO–injected embryos. Hapln1b morphants displayed 3 phenotypes: normal, mild, and severe. (E) ISH expression of mfap4, mpx, and gata1 in control MO– or hapln1b MO–injected embryos. H1b, hapln1b. (F) Imaging double-transgenic kdrl:mCherry/cmyb:GFP embryos at 36 hpf. Bar represents 50 µm. Control (ctl) MO vs hapln1b mRNA injected, P < .0001. Student unpaired t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Blood Adv