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Fig. 1

ID

ZDB-FIG-220629-23

Publication

Roca et al., 2022 - Tumor necrosis factor induces pathogenic mitochondrial ROS in tuberculosis through reverse electron transport

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Fig. 1

Fig. 1. ETC-derived mROS drive necrosis of Mm-infected macrophages in TNF-high conditions.

(A) Representative pseudocolored confocal images of wild-type or TNF^hi larvae with yellow fluorescent protein (YFP)–expressing macrophages (green), 1 day after infection with EBFP2-expresssing Mm (blue), showing MitoTracker Red CM-H₂Xros (magenta) fluorescence. White arrowheads, uninfected macrophages; yellow arrowheads, infected macrophages; yellow arrows, infected macrophages positive for mROS. Scale bar, 20 μm. (B) Quantification of mROS in wild-type or TNF^hi larvae 9 hours after injection of live or heat-killed Mm. Each point represents the mean maximum intensity fluorescence of MitoTracker Red CM-H₂Xros per fish. Black symbols represent macrophages that do not contain bacteria. Red and purple symbols represent Mm-infected and heat-killed Mm-containing macrophages, respectively, in the same animal. Horizontal bars denote means; *P < 0.05 (one-way ANOVA with uncorrected Dunn’s post-test for differences between macrophages in the same animal and with Tukey’s post-test for differences between treatments). Data are representative of two independent experiments. (C to F) Quantification of mROS in larvae 1 day after infection with Mm that are wild-type or TNF^hi treated with FCCP (C), DNP (D), nigericin (E), or diazoxide (F), or vehicle. Horizontal bars denote means; ****P < 0.0001 (one-way ANOVA with Tukey’s post-test). Data are representative of two or three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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