FIGURE

Fig. 3 - supplement 1

ID

ZDB-FIG-220621-23

Publication

Amini et al., 2022 - Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina

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Fig. 3 - supplement 1

Analysis of cell and nuclear deformations of HC during migration.

(A) Cytoplasmic deformations of a horizontal cell (HC) migrating from the basal inner nuclear layer (INL) to the HC layer. Cells from Tg(lhx-1:eGFP) transgene embryo were transplanted into wild-type (WT) embryos. White line: apical surface; red dot: tracked HC. Scale bar: 10 μm. Frames from Video 4. (A’) Close-up of HC in (A). Scale bar: 5 μm. (A’’) Representative sequences of the automated segmented contours of the cell body (yellow line) generated by Icy. (B–D) Cell morphodynamic changes in 12 HCs from seven embryos; (B) perimeter (μm), (C) elongation ratio, (D) sphericity (%). See Figure 3—source data 2. (E-E’) HCs nuclei undergo deformations and exhibit different shapes as they move to their final position. Nuclear envelopes are labeled by lap2b:eGFP (gray), and HCs/PRs are labeled by trβ2:tdTomato (magenta) DNA constructs. Dashed line: outer plexiform layer (OPL); red arrowhead: tracked HC; scale bar: 10 μm. (E’) Close-up of HC in (E) showing nuclear envelopes (gray). Scale bar: 5 μm. Time in h:min (A, E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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