ZFIN Image: Amini et al., 2022, Fig. 3

Image

Figure Caption

Fig. 3 - supplement 1

Analysis of cell and nuclear deformations of HC during migration.

(A) Cytoplasmic deformations of a horizontal cell (HC) migrating from the basal inner nuclear layer (INL) to the HC layer. Cells from Tg(lhx-1:eGFP) transgene embryo were transplanted into wild-type (WT) embryos. White line: apical surface; red dot: tracked HC. Scale bar: 10 μm. Frames from Video 4. (A’) Close-up of HC in (A). Scale bar: 5 μm. (A’’) Representative sequences of the automated segmented contours of the cell body (yellow line) generated by Icy. (B–D) Cell morphodynamic changes in 12 HCs from seven embryos; (B) perimeter (μm), (C) elongation ratio, (D) sphericity (%). See Figure 3—source data 2. (E-E’) HCs nuclei undergo deformations and exhibit different shapes as they move to their final position. Nuclear envelopes are labeled by lap2b:eGFP (gray), and HCs/PRs are labeled by trβ2:tdTomato (magenta) DNA constructs. Dashed line: outer plexiform layer (OPL); red arrowhead: tracked HC; scale bar: 10 μm. (E’) Close-up of HC in (E) showing nuclear envelopes (gray). Scale bar: 5 μm. Time in h:min (A, E).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife