FIGURE
Fig. 5
- ID
- ZDB-FIG-220621-15
- Publication
- Liu et al., 2022 - LAPTM4B-35 promotes cancer cell migration via stimulating integrin beta1 recycling and focal adhesion dynamics
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Fig. 5
LAPTM4B-35 promoting recycling of integrin beta1. A, Western blotting shows recycled integrin beta1 to be reduced in KO cells and rescuable by LAPTM4B-35 expression. Left panel: a representative experiment. Right panel: quantification of three experiments, mean ± SEM, p(WT, KO) = 0.044, p(KO, LAPTM4B-35) = 0.024. B, LAPTM4B KO cells stably expressing LAPTM4B-35-Flag or LAPTM4B-24-Flag, together with A431-expressing LAPTM4A-Flag cells, were pulled down via anti-Flag antibody; coimmunoprecipitated integrin beta1 detected by Western blotting. C, A431 cells stably expressing LAPTM4B-35-mCherry were stained with anti-integrin beta1(N29) antibody. Confocal microscopy revealed the colocalizations to be on the endosome. Scale bar: 10 ?m. Yellow arrows highlight the colocalization. D, A431 cells stably expressing LAPTM4B-35-GFP were transiently transfected with integrin beta1-mCherry and imaged by live TIRF microscopy. Scale bar: 10 ?m. A magnified region of interest at different time points is displayed on the right. Yellow arrows highlight the colocalization
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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