Fig. 4

Exogenous lactate accelerates the caudal fin regeneration after amputation. (A) Graph showing the fin growth length of uncut larvae at 144 hpf when immersed in zebrafish water (CT), or when immersed in lactate (mean ± SEM, n < 30, Mann Whitney test, two-tailed, non-significant). (B) Representative images of caudal fin regeneration at 72 hpA (Scale bar = 100μm) with the corresponding graph (C) showing the fin length after amputation and immersion with zebrafish water (CT), lactate (100 μM) or GAL (500 nM) and lactate (100 μM) added at the same time (mean ± SEM, n < 30, Mann Whitney test, two-tailed, ***p ≤ 0.001). (D) Graph showing the fin area after amputation and immersion with zebrafish water (CT), lactate (100 μM) or GAL (500 nM) and lactate (100 μM) added at the same time at 72 hpA (mean ± SEM, n < 30, Mann Whitney test, two-tailed, ****p ≤ 0.0001).