Fig. 8

a 100?µM A-438079 (A43) treatment decreased hyperactivity in TL in the first 10?min of PTZ treatment (?pixel?±?s.e.m.; n?=?46; P?=?2.1?×?10^?3). The dashed gray line indicates TL?s max average activity score treated with only PTZ (n?=?36). b AUCs for TL?s baseline activity and A43 treatment did not differ (open points; P?=?0.9). A43 treatment significantly reduced the effect of PTZ without (filled points; P?=?2.4?×?10^?3) and with (P?=?4.5?×?10^?2) extracted baseline activity. c Stage III count (count/2?min) was significantly reduced with A43 treatment in TL (n?=?18; P?=?<?1.0?×?10^?4). Dashed lines represent max average Stage II and Stage III counts for TL?s PTZ-only group. d A43 treatment decreased PTZ-induced hyperactivity in panx1b^?/? (n?=?46; P?=?4.0?×?10^?4). The dashed magenta line indicates panx1b^?/??s max average activity in the PTZ only group (n?=?31). e A43 treatment significantly reduced the effect of PTZ in panx1b^?/? without (filled points; P?=?5.0?×?10^?4) and with (P?=?8.7?×?10^?3) extracted baseline activity. f Stage II and III counts were significantly reduced with A43 treatment in panx1b^?/? (n?=?18; stage II: P?=?1.0?×?10^?4; stage III: P???1.0?×?10^?4). Dashed lines represent max average Stage II and Stage III counts for panx1b^?/??s PTZ-only group. g SOM revealed changes to the PTZ phenotype in TL with A43 treatment (n?=?46) for freezing and normal locomotor behaviors (P?<?0.0001). A43 treatment resembled panx1a^?/??s PTZ phenotype the most, and panx1b^?/??s the least, as seen in normal (mid count P?<?0.0001) and bursting behaviors (P?<?0.0001). Smaller value and warmer color represent higher similarity. Statistical tests used: Two-way repeated measures ANOVA and Bonferroni multiple comparisons for(a, c, d & f); Mann?Whitney U test for (b and e). Fisher?s exact test for (g). N?=?number of larvae. *P?<?0.05, **P?<?0.01, ***P?<?0.001, ****P?<?0.0001.