Fig. 1

a Workflow for recording in vivo local field potentials (LFP) from the right optic tectum (OT) of 7dpf anesthetized larva after 15?mM PTZ treatment. b Representative recording of baseline activity and a seizure-like event (SLE) induced with the addition of PTZ from a TL larva. c All TL and panx1b^?/? larvae had SLEs (TL (gray) n?=?7/7; DKO (light blue) n?=?4/12, P?=?4.5?×?10^?3; panx1a^?/? (deep blue) n?=?1/8, P?=?3.0?×?10^?4; panx1b^?/? (magenta) n?=?9/9, P?=?1.0, Barnard test). d Quantification of SLEs in the first hour of LFP recording revealed that all panx1 knockout (KO) larvae had a significant reduction in SLEs compared to PTZ treated TL controls, presented as an average number of SLEs/hour?±?s.e.m. (DKO: P?=?<?1.0?×?10^?4; panx1a^?/?: P?=?3.0?×?10^?4; panx1b^?/?P?=?5.7?×?10^?3). e The average duration of SLEs (in seconds?±?s.e.m.) for each genotype was not significantly different compared to TLs. Statistical tests were not significant for panx1a^?/? due to lack of statistical power. (DKO: P?=?0.07; panx1b^?/?: P?=?0.09). f All panx1 knockout larvae had a significant delay in the average onset time (minutes?±?s.e.m.) of the first seizure-like event compared to PTZ treated TL controls. (DKO: P?=?6.1?×?10^?3; panx1b^?/?: P?=?3.0?×?10^?4). g All panx1 knockout larvae spent significantly less time seizing compared to PTZ treated TL controls. Average fractional time spent seizing is presented in percent?±?s.e.m. (DKO: P?=?<?1.0?×?10^?4; panx1a^?/?: P?=?3.0?×?10^?4; panx1b^?/?: P?=?0.01). N?=?number of larvae. Statistical Mann?Whitney U test in (d?g). Scale bars 0.5?mV by 0.5?s. *P?<?0.05, **P?<?0.01, ***P?<?0.001, ****P?<?0.0001.