FIGURE

Figure EV3

ID

ZDB-FIG-220516-6

Publication

Karabid et al., 2022 - Angpt2/Tie2 autostimulatory loop controls tumorigenesis

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Figure EV3

Tie2 on PitNET cells is phosphorylated and can be stimulated by Angpt2

Co‐IF for both P‐Tie2 (Tyr 1102/1108; red) and for Na⁺K⁺ ATPase (green), used as plasma membrane marker, of GH3 cells transduced with unspecific shRNA (shCtrl) and shAngpt2 (#2) and incubated with rhANGPT2 or left untreated. Original magnification: 400×; scale bar: 20 μm.

Quantification of P‐Tie2 immunostaining intensity in cells shown in (A) shCtrl‐transduced GH3 cells (266.71 ± 12.12); in shAngpt2 cells (176.04 ± 8.46); in control cells treated with rhANGPT2: shCtrl + rhANGPT2: 246.33 ± 9.79 (versus shCtrl, not significant by t‐test); in treated knockdown cells: shAngpt2 + rhANGPT2: 218.68 ± 18.16 (versus shC2; *P = 0.0421 by t‐test). The experiment was performed twice each with three technical replicates. Intensities are expressed as arbitrary units ± SEM. ***P < 0.0001 by t‐test.

Tie2 and P‐Tie2 expression in serum‐starved GH3 cells transfected with siAngpt2 POOLs and stimulated with rhANGPT2 for the indicated times.

Tie2 and P‐Tie2 expression in cells as in (C) stimulated with the indicated doses of rhANGPT2 for 30 min.

Co‐IF for Angpt2 (red) and P‐Tie2 (green) of a representative rat primary NF‐PitNET. Nuclei were counterstained with DAPI. White arrows point to Angpt2‐positive (P‐Tie2 negative) cells in adjacent non‐tumor area. Scale bars: 50 µm. T, tumor area.

Data information: (C, D) Blots shown in all panels are representative of three independent experiments. The numbers represent the ratio phospho/total proteins.

Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.