FIGURE

Fig. 3

ID

ZDB-FIG-220511-24

Publication

Nagabhishek et al., 2019 - A marine sponge associated fungal metabolite monacolin X suppresses angiogenesis by down regulating VEGFR2 signaling

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Fig. 3

(A) Monacolin X inhibited endothelial cell sprouting in an aortic ring assay. Aortas were harvested from 12 day old chick and cut into 1 mm slices, which were then placed in 12-well plates containing matrigel. The rings were photographed and analyzed. The endothelial cell sprouting was abundant in the control aortic rings (A) but not in the rings treated with monacolin X and SU5416 suppressed the endothelial cell tube formation. All the experiments were done with the presence of VEGF (20 ng mL⁻¹), (B) shows graphs for number of sprouts and length of capillary sprouts (μm). (C) Representative vascularization of the chorioallantoic membrane (CAM Assay) following 8 h of incubation with monacolin X at (15 μM, 30 μM and 60 μM) and compared to control and positive control (VEGF 20 ng mL⁻¹). (D) shows the mean vessel area as a percentage of the total area, mean vessel length and mean number of branch points were obtained by Wimasis – Wimcam software. Three independent experiments were performed and the results were taken under (10×) magnification and. Each value was presented as means + SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 compared with control (one-way ANOVA).

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Acknowledgments

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